Figure 4.
The GTPase activity of l-Mgm1 is not essential for fusion in vivo. (A) Schematic of in vivo l- and s-Mgm1 constructs. Isoforms were constructed by deletion of the first hydrophobic domain (HD1) to produce s-Mgm1 and the second hydrophobic domain (HD2) to produce l-Mgm1. l-Mgm1S224A is functional in vivo as assessed by growth of yeast strains on glycerol media (B) and by mitochondrial morphology (C). Bar, 1 µm. A quantification of mitochondrial morphology in Δmgm1 cells expressing the indicated l- and s-Mgm1 combinations is shown. Data were normalized to Δmgm1 + l-Mgm1 + s-Mgm1 and are shown as the mean + SEM (error bars; three experiments, >50 cells/experiment).