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. 2009 Sep 21;186(6):897–914. doi: 10.1083/jcb.200902096

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© 2009 Sumakovic et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 10. — The RAB-2 GTP-bound form recruits RIC-19 to the Golgi. (A) When expressed in HeLa cells, RIC-19 coimmunoprecipitates with wild-type (WT) RAB-2 and its GTP-bound RAB-2(Q65L)DA form but not its inactive GDP-bound RAB-2(S20N)DN form. As a control, the amount of affinity-purified RAB-2 is shown below. IP, immunoprecipitation. (B) Endogenous RIC-19 levels are not altered in unc-108 mutants, as determined by Western blotting total extracts. (C) RIC-19 localization is mostly cytoplasmic in cells expressing the inactive RAB-2(S20N)DN, but RIC-19 is recruited to Golgi membranes by expression of either wild-type RAB-2 or, to a greater extent, GTP-bound RAB-2(Q65L)DA. Arrowheads show the recruitment of RIC-19 by overexpression of GTP-restricted (DA) RAB-2. (D) As compared with wild-type motor neurons, RIC-19–mYFP shows increased Golgi recruitment in the dominant unc-108 mutants (marked by arrowheads). Bars, 4 µm.