Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Sep 21;186(6):915–928. doi: 10.1083/jcb.200905065

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Nowak et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Mouse lenses lacking Tmod1 have decreased levels of a short γTM, whereas global actin levels are unchanged. (A) Western blots of Tmod1, actin, γTM, and GAPDH in cortical fiber cell extracts from 2-mo-old mice. Each lane is 1/4 of a lens cortex from a different animal. (B) Quantification from blots in A, normalized to GAPDH. γTM is reduced ∼3-fold (P < 0.002), but actin is unchanged (P < 0.96). n = 4 animals per genotype. (C) Western blots of Tmod1, actin, and γTM in total cortical fiber cell extracts (T), cytosol (C), and membranes (M) from 4-mo-old mice. Each lane is 1/10 of a lens cortex. (D) Percentage of membrane-associated Tmod1, actin, or γTM. Percentage of γTM on membranes is ∼60% in Tmod1+/+^Tg+ and ∼30% in Tmod1−/−^Tg+ lenses (P < 0.008). Actin levels on membranes are unchanged (P < 0.2). n = 3 experiments.

HHS Vulnerability Disclosure