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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Aug 18;877(27):3169–3174. doi: 10.1016/j.jchromb.2009.08.011

Figure 2. Comparison of ELISA and LC/MS/MS analysis of LTE4 in urine from asthmatics.

Figure 2

Urine from 10 asthmatic subjects was obtained, centrifuged within 15 hours, and processed as follows: One aliquot was analyzed in-house using LC/MS/MS on the day of collection (LCMS-1) and several aliquots were frozen at -80°C immediately after collection. One frozen aliquot was analyzed by Cayman using ELISA (ELISA) according to the manufacturer's protocol and a frozen aliquot was analyzed in-house using LC/MS/MS (LCMS-2). Creatinine levels were obtained from Cayman and used to normalize all data. For LC/MS/MS samples were injected in duplicate and their average and CV were determined. CV values for ELISA were obtained from Cayman. Methods used are on the x-axis and Panel A shows normalized LTE4 values in pg/mg creatinine (y-axis) and Panel B shows CVs (y-axis).