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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Aug 18;877(27):3169–3174. doi: 10.1016/j.jchromb.2009.08.011

Table 1. Analysis of spiked pooled urine samples from healthy adults by enzyme immunoassay.

Method 1- Loose Sorbent 2- Sorbent Column 3- HLB SPE cartridge 4- Off-line HPLC
Spike Amount
(pg/mL)		 Ave Conc CV %R Ave Conc CV %R Ave Conc CV %R Ave Conc CV %R
0 17.5 75 NA 133.6 * NA 147.9 13.8 NA 27.5 10 NA
15.6 36.7 4.2 123.4 198.3 * 414.7 148.2 25.1 2.2 36.7 13.1 59.3
62.5 38.8 8 34.2 224.7 * 145.8 262.1 7.9 182.7 107.3 8.8 127.7
250 197.1 11.4 71.9 69.3 * -25.7 489.4 6.3 136.6 216.1 5 75.5

Pooled urine samples (n=2) were spiked with 0, 15.6, 62.5, or 250 pg/ml of LTE4 and then purified using the indicated method, followed by EIA analysis.

Method 1=Cayman loose sorbent

Method 2=Cayman sorbent columns

Method 3=HLB SPE cartridge

Method 4=Off-line HPLC

*

Precision data was not obtained