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a. Representative traces showing cleavage in presence of enzyme ( panel 1) and no cleavage in absence of enzyme (panel 2) b. Enzyme kinetics. The hyperbolic dependence of catalytic rate on enzyme concentration was fit with the single-molecule Michaelis-Menten equation (21) (solid line). Data points and standard error were determined from single parameter exponential fits to the survival fraction as a function of time (inset).
