Skip to main content
. Author manuscript; available in PMC: 2009 Sep 28.
Published in final edited form as: Gastroenterology. 2008 May 22;135(3):947–955.e5. doi: 10.1053/j.gastro.2008.05.046

Fig. 5. AR suppresses p53 and down-stream target genes.

Fig. 5

(A) p53 protein expression in 36-weeks DEN-induced mouse HCC livers. We measured p53 expression with immunoblotting and show in our quantitative results that p53 expression in the AR+/y mice is lower than L-AR−/y. GAPDH served as loading control. (B) AR, p53 and p21 protein expression in 36-weeks DEN-injected mouse normal livers. Immunoblotting demonstrates thatp53 expression in AR+/y mice is lower than that in L-AR−/y. β-Actin served as loading control. Higher expression of p53 and p21 proteins were detected in L-AR−/y livers compared to AR+/y (n=4 of each group). (C) We examined Gadd45α and β protein expression using specific antibodies, and compared AR+/y and L-AR−/y liver tumors. Quantitation of Gadd45α and β protein expression in lower panel. * indicates significant difference in AR+/y and L-AR−/y (p<0.05).