Skip to main content
. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2009 May;68(5):515–524. doi: 10.1097/NEN.0b013e3181a24b53

Figure 3.

Figure 3

Measurement of 〈-synuclein and tyrosine hydroxylase (TH) expression in midbrain tissue 6 weeks post-viral injection. Western blots show samples from midbrain ipsilateral to 〈-synuclein (wild type [wt], S129A [A], and S129D [D]) injection and are compared to protein extracts from the contralateral midbrain injected with empty vector (−) control in the same animal. Protein from left and right midbrain samples was probed with primary antibody to 〈-synuclein and TH, followed by secondary HRP-conjugated antibody and ECL detection. Bands were quantified and normalized to GAPDH loading control. The ratio of 〈-synuclein and TH for the α-synuclein (ipsilateral) versus empty vector (contralateral) side was calculated for each animal. Blots show 〈-synuclein overexpression for most animals, but there was variable expression as shown for the 2 wt samples. Decreased TH expression is also shown for wt and S129 mutants.