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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Hum Pathol. 2008 Oct 29;40(3):341–348. doi: 10.1016/j.humpath.2008.08.006

Figure 5.

Figure 5

Ethidium-stained gels of the RT-PCR results (a separate gel was run for each patient at the time of diagnostic assay). Total RNA was extracted from formalin-fixed, paraffin-embedded tumor tissue and subjected to RT-PCR using a consensus PAX3/PAX7 primer and FOXO1 primer. M, DNA molecular weight marker (100 bp ladder); Lane 1 in each case represents the respective patient sample (PAX3-FOXO1 fusion product is 172 bp and PAX7-FOXO1 fusion product is 160 bp); Lane 2, PAX3-FOXO1 positive control; and Lane 3, no template negative control.