Table III. Cytokines produced in cultures of SEA-stimulated blood mononuclear cells from study subjects: association of disease with IL-10 and RANTES.
The number of subjects in each group is indicated in Table II. Cytokines were titrated after 20 h (TNF-α, IL-10, IL-1α, RANTES, and MCP-1) or 72 hours (IL-4, IL-13, IL-5, IL-6 and MIP-1α) of culture. Cytokine levels were compared between the clinical groups, using the non-parametric Mann-Whitney test; statistically significant differences were observed for IL-10 and RANTES (**p=4 × 10-4) and MIP-1α (*p=7 × 10-3) when comparing patients with no or mild PPF with the group with severe PPF.
|
No or Mild PPF
grade A, B, C,CLL |
Intermediate PPF
grade CLM |
Severe PPF
grade CLH, D, E |
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|---|---|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||||
| Culture conditions | Detection limit | % >limit | Median values (pg/ml) | 75% upper percentile value | % >limit | Median values (pg/ml) | 75% upper percentile value | % >limit | Median values (pg/ml) | 75% upper percentile value | |
| TNF-α | SEA 20h | 3.9 | 42 | 19.4 | 432 | 17 | 148.5 | 476 | 8 | 58.9 | 386 |
| IL-10** | SEA 20h | 3.9 | 95 | 151.6 | 240 | 91 | 166.6 | 279 | 67 | 34.7 | 145 |
| IL-4 | SEA 72h | 3.9 | 32 | 2 | 6 | 33 | 2 | 9 | 39 | 2.4 | 13 |
| IL-13 | SEA 72h | 0.78 | 94 | 14.2 | 98 | 96 | 12.3 | 165 | 78 | 16.1 | 142 |
| IL-1α | SEA 20h | 3.9 | 78 | 20.1 | 35 | 80 | 16.9 | 36.3 | 59 | 7.7 | 49 |
| IL-5 | SEA 72h | 3.9 | 42 | 4 | 84 | 43 | 4 | 77 | 50 | 5 | 95 |
| IL-6 | SEA 72h | 1.5 | 100 | 4106.2 | 9081 | 100 | 5526.3 | 11363.5 | 100 | 2917.2 | 7592 |
| IFN-γ | SEA 72h | 0.78 | 84 | 19.7 | 72 | 75 | 18.3 | 52 | 83 | 19.8 | 46 |
| RANTES** | SEA 20h | 7.8 | 100 | 7097.1 | 10472 | 100 | 5953.4 | 8765 | 100 | 3331.4 | 5127 |
| MIP-1a* | SEA 72h | 3.9 | 100 | 7053.7 | 9505 | 100 | 5432 | 15762 | 100 | 1802.3 | 8421 |
| MCP-1 | SEA 20h | 3.9 | 100 | 22799 | 37093 | 100 | 38499 | 68852 | 100 | 32889 | 59146 |