Figure 8.
Antiviral activity of tamarin IFNγ in tamarin hepatocytes infected with GBV-B. A. Pre-infection IFNγ treatment. Tamarin primary hepatocytes were treated with various concentration of tamarin IFNγ (0 to 5.0 ng/ml) for 24 hr prior to infection with GBV-B and for an additional 72 hr after infection. Total RNA was purified from cells and media and analyzed for GBV-B RNA genome equivalents (GE) by quantitative RT-PCR. B. Post-infection IFNγ treatment. Tamarin hepatocytes were infected with GBV-B for 3 days to establish the infection and then treated with tamarin IFNγ (+) at 5.0 ng/ml for 1, 2, 3 or 7 days before harvest. The Δ symbol denotes that the sample was below the level of detection. Error bars indicate the variation in values from duplicate cultures. The level of variation was not sufficient to yield visible error bars on those were none appear.