Table 2.
Mutational analysis of the CAN1 coding region Wt and rev3 Strainsa
| Type of Mutationb | Wt Control |
Wt 10 mM Cr(VI) |
rev3 Control |
rev3 10 mM Cr(VI)‡ |
|---|---|---|---|---|
| Transitions | 75.9% (22) | 61.3% (19) | 90.0% (36) | 82.4% (27) |
| GC→AT | 27.6% (8) | 19.4% (6) | 32.5% (13) | 32.4% (11) |
| AT→GC | 48.3% (14) | 41.9% (13) | 57.5% (23) | 50.0% (17) |
| Transversions | 24.1% (7) | 22.6% (7) | 10.0% (4) | 17.6% (6) |
| GC→TA | 13.8% (3) | 6.5% (2) | 2.5% (1) | 5.9% (2) |
| GC→CG | 3.4 (1) | 6.5% (2) | 0 | 0 |
| AT→CG | 6.9% (2) | 0 | 0 | 8.8% (3) |
| AT→TA | 3.4% (1) | 9.7% (3) | 7.5% (3) | 2.9% (1) |
| Deletions | 0 | 12.9% (4) # | 0 | 0 |
| Insertions | 0 | 3.2 (1) | 0 | 0 |
a
Data from 10 independent mutant clones for each strain/condition
b
Mutational analysis of CAN1 coding region
Parenthesis indicate the number of mutants
#
Cr(VI)-induced deletions were significantly different from WT control (P=0.052), and rev3 10 mM (P=0.049) yeast.
‡
The mutational spectra generated by Cr(VI) in WT and rev3 yeast were significantly different (P=0.013).