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. Author manuscript; available in PMC: 2009 Sep 28.
Published in final edited form as: Cell. 1988 Sep 23;54(7):1081–1090. doi: 10.1016/0092-8674(88)90123-7

Figure 4. Synthetic Version of the Consensus Sequence.

Figure 4

(A) Different arrangements of a synthetic consensus sequence. A 12 bp nearly palindromic sequence (NP), TCAATTAAATGA, was synthesized. Positions 1 through 10 of this sequence represent the consensus sequence. The G and A (positions 11 and 12) were added in order to create a BcII site at the junction between two consensus sequences. The arrows indicate the orientations of the consensus sequences in cloned repeats. Note that the addition of the G and A at positions 11 and 12 creates a sequence in the opposite polarity that matches the consensus at 9 out of 10 positions. One or several copies of the NP sequence were cloned in various orientations within the BamHI site of the M13mp18 polylinker.

(B) The NP sequence is nearly palindromic, imperfect at positions 4 and 9, which are both A. Palindromic sequences were synthesized by duplicating in the opposite polarity the left six bases (left palindrome, LP) or duplicating the right six bases (right palindrome, RP) of the NP sequence. Various numbers of copies of the LP and RP sequences were cloned into the BamHI (LP1, LP3, and RP sequences) or Smal (LP2) sites of M13mp18.