| Component | Volume per reaction (μl) | Final concentration or amount |
|---|---|---|
| forward oligonucleotide (1.0 mg/ml) | 1.0 μl | 1.0 μg |
| reverse oligonucleotide (1.0 mg/ml) | 1.0 μl | 1.0 μg |
| 1× TE buffer | 18 μl | 18 μl |
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