Fig. 1. lgl germline clones affect oocyte polarity.

(A,B) Stau was localized at the posterior in the wild type (A) but is mislocalized in lgl⁴ mutant germline clones (B). (C,D) Grk and the oocyte nucleus (arrow) were localized to the anterior corner (C) but were mislocalized to a lateral position in some lgl⁴ mutant germline clones (D). (E,F) osk mRNA in wild-type egg chambers showed a posterior localization at stage 10 (E), whereas lgl⁴ germline clones displayed clear mislocalization of osk mRNA to the center of the oocyte (F). (G,H) bcd mRNA in wild-type egg chambers showed disc-shaped localization in the oocyte anterior (G, arrow), but in lgl⁴ germline clones bcd sometimes accumulated as dots at the anterior of the oocyte (H, arrow). (I,J) Kin:β-Gal showed posterior localization in the wild type (I) but was mislocalized to the center of the oocyte in lgl⁴ germline clones (J). (K,L) α-Tubulin showed an anterior-to-posterior gradient in the wild-type oocyte (K) but was uniformly distributed in lgl⁴ germline clones (l). Mutant germline clones were marked by the absence of GFP (green) in the nuclei of the nurse cells (B,D,J) or were generated by the dominant female sterile method (F,H,L). Anterior is towards the left and posterior is towards the right in all panels.