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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Chem Res Toxicol. 2009 May;22(5):818–823. doi: 10.1021/tx800392j

Figure 3.

Figure 3

Michael-Menten plots used to determine Vmax and Km for the X/XO reduction of NO in the presence of quinones. Samples were N2-saturated solutions containing 20 μM NO, 10 mU XO/mL, 50 μM X, and 50 mM phosphate buffer (pH 7.4) and in the presence of 3.33-20 μM quinone. The temperature is 37 °C. Initial rates of NO consumption used were those obtained after subtracting the basal initial rate, that is, in the absence of quinone, (6.2 ± 0.3) × 10-8 M/s’ from the measured initial rate. Error bars are standard errors of the mean value of at least three determinations.