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. Author manuscript; available in PMC: 2010 Jul 28.

Published in final edited form as: Biochemistry. 2009 Jul 28;48(29):6835–6845. doi: 10.1021/bi900633p

Binding of PI-PLC variants to PG/PC SUVs as a function of X_PC. The apparent K_d values were measured by FCS for variants labeled with AF488 at N168C: *N168C (●), *P42G (▲), *K44A (■), *K44E (❒), *Y88A (), and *3YS (❍). The two dotted lines indicate X_PC = 0.2 and 10 mM total phospholipids. The error bars are the standard deviations of K_d from two independent protein and SUV preparations.

Inline graphic — Binding of PI-PLC variants to PG/PC SUVs as a function of X_PC. The apparent K_d values were measured by FCS for variants labeled with AF488 at N168C: *N168C (●), *P42G (▲), *K44A (■), *K44E (❒), *Y88A (), and *3YS (❍). The two dotted lines indicate X_PC = 0.2 and 10 mM total phospholipids. The error bars are the standard deviations of K_d from two independent protein and SUV preparations.