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. Author manuscript; available in PMC: 2010 Jul 28.

Published in final edited form as: Biochemistry. 2009 Jul 28;48(29):6835–6845. doi: 10.1021/bi900633p

Relative activities of PI-PLC enzymes toward PI/PC SUVs. (A) Activities at fixed X_PC = 0.2 with different total phospholipid concentrations for wildtype () and Y88A (///) enzymes. (B) Relative activities, compared to wildtype activity towards pure PI SUVs, of wildtype (●), K44A (■), Y88A (), and 3YS (❍) towards 10 mM PI in the presence of increasing concentrations of PC. (C) Relative activity of wildtype (●), data taken from reference (15), and K44A (■) at fixed total phospholipid (10 mM) with varying X_PC. Assays for K44A were carried out in duplicate; the 15% error bars represent the maximum error for these assays.

Inline graphic — Relative activities of PI-PLC enzymes toward PI/PC SUVs. (A) Activities at fixed X_PC = 0.2 with different total phospholipid concentrations for wildtype () and Y88A (///) enzymes. (B) Relative activities, compared to wildtype activity towards pure PI SUVs, of wildtype (●), K44A (■), Y88A (), and 3YS (❍) towards 10 mM PI in the presence of increasing concentrations of PC. (C) Relative activity of wildtype (●), data taken from reference (15), and K44A (■) at fixed total phospholipid (10 mM) with varying X_PC. Assays for K44A were carried out in duplicate; the 15% error bars represent the maximum error for these assays.