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. Author manuscript; available in PMC: 2010 Jul 28.
Published in final edited form as: Biochemistry. 2009 Jul 28;48(29):6835–6845. doi: 10.1021/bi900633p

Figure 6.

Figure 6

Fluorescently labeled PI-PLC protein (3.5 nM) bound to vesicles as a function of total lipid concentration with (1:1) PG/PC SUVs: *N168C with 0.02 µM (❍), 0.57 µM (●) or 5.7 µM (X) unlabeled wildtype PI-PLC added. The points shown are from two different titrations with different enzyme preparations and different vesicle preparations. (B) Variation in apparent Kd for 3.5 nM *N168C binding to PG/PC (1:1) SUVs with unlabeled PI-PLC concentration.