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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Orthod Craniofac Res. 2009 Aug;12(3):178–186. doi: 10.1111/j.1601-6343.2009.01451.x

Figure 2.

Figure 2

Regulation of human MMP-1 gene promoter by relaxin without or with β-estradiol requires the −139/−67 bp motif. Cell lysates from cells transiently transfected with the −1200/−42 or −139/−42 or −66/−42 bp human MMP-1 promoter-CAT constructs (A) and exposed to control medium (Ct) or medium containing β-estradiol (Es) and/or relaxin (R) or TPA were assayed for β-gal and CAT. (B) The histogram for mean (±SE) fold-induction of CAT/β-gal for each treatment relative to controls was plotted for four experiments. (C) Gelatin-substrate zymography on cell-conditioned medium was used to visualize the changes in levels of endogenously expressed presumptive MMP-1 (arrow). (*p < 0.05, **p < 0.005, ***p < 0.0005).