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. 2009 Aug 25;10:397. doi: 10.1186/1471-2164-10-397

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Copyright © 2009 Williams et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Read-through transcription of M. leprae pseudogenes. This figure represents the results of RT-PCR analysis of transcriptional read-through between M. leprae pseudogenes and their upstream ORFs. Panel A shows mapped genomic regions where pseudogenes (ML0832, ML1483c, ML0179c, and ML0091c) and upstream ORFs are located http://genolist.pasteur.fr/Leproma/. Black arrows indicate direction of transcription. Small red arrows indicate forward and reverse primers for RT-PCR. Red line below primers and (bp) designate size of predicted PCR product if genes are expressed as a polycistronic mRNA. Panel B shows ethidium bromide-stained agarose gel analysis of these PCR amplicons: Lane 1, 100 bp DNA ladder (New England Biolabs); Lane 2, PCR amplicons from nu/nu footpad-derived M. leprae Thai-53 cDNA for each gene set; Lane 3, RT (-) control; Lane 4, 1 ng M. leprae DNA (positive control).