Figure 8. Allatostatin-induced attenuation of V3 neuronal activity in adult Sim1^Cre; AlstR192 mice.

Kinematic analysis of Sim1^Cre; AlstR192 mice before and after application of allatostatin ligand to the lumbar spinal cord. ACSF alone (left) and 1mM allatostatin in ACSF (right) were applied to the exposed spinal cord (L2-L4 segments) of a Sim1^Cre; AlstR192 mouse.

Upper panels: Stick diagrams illustrating the stance and swing movements of the left hindlimb. Allatostatin application causes a disordered gait as illustrated by the increased variability in timing and phasing of stepping movements. Middle panels: Foot placement analysis during the same walking sequence, which shows increased variability in the positioning of the hind paw following application of allatostatin. Note the meandering trajectory following application of allatostatin. Lower panels: Stance phase (solid bars) and swing phase (empty bars) for the left (L) and right (R) hindlimbs during the same bout of locomotion. Application of allatostatin causes a marked disruption in the duration and timing of the stance and swing phases. Periods of substantial overlap between the stance (extensor) phase of the left and right hindlimbs are indicated by a double asterisk. Skipping movements involving overlapping swing (flexor) phases for the left and right hindlimbs are indicated by a chevron.