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. Author manuscript; available in PMC: 2009 Sep 28.

Published in final edited form as: Nat Med. 2008 Jun 29;14(7):778–782. doi: 10.1038/nm1785

(a) Bile was collected from the common bile duct of 5-6 month old mice. Cholesterol (CH) secretory rates were derived from cholesterol concentrations and normalized to body weight (BW, *p=0.003, n=4-5 per group). (b) Relative lipid compositions (mol per 100 mol) of gallbladder bile obtained from Lox (open circles) and LIRKO (closed triangles) mice fed a chow diet were calculated and plotted on a partial condensed phase diagram (see Methods). The different phases are indicated for equilibrium conditions: micelles only (yellow); micelles and liquid crystals (orange); micelles, liquid crystals and cholesterol monohydrate crystals (red); and micelles and cholesterol monohydrate crystals (gray). Relative lipid compositions of bile from control mice plot in a zone in which only micelles would form at equilibrium, but bile from LIRKO mice plot in zones in which liquid crystals (orange) or both liquid crystals and cholesterol monohydrate crystals (red) in addition to micelles would form at equilibrium. (c) Cholesterol Saturation Indices (CSI) of gallbladder bile obtained from 5-6 month old mice were calculated as described in Methods. (d) Gallbladder volumes from 5-6 month old mice. Each point represents a single mouse, whereas long and short lines represent mean and SEM, respectively (*p=0.004). (e) Cholesterol absorption was measured in 4 month old mice by a fecal dual isotope ratio method. (f) Hepatic lipids were extracted from 2-3 month old Lox and LIRKO mice and total cholesterol content was measured by gas chromatography (n=4-6, *p<0.05). (g) Real-time PCR analysis of RNA isolated from the livers of non-fasted 8-10 week old mice (n=8, *p<1 x 10^-5). (h) Membranes purified from 8-10 week old mice were subjected to immunoblotting with antibodies against ABCG5 or ABCG8, and quantified with ImageJ software (*p<0.05, n=3-4).

(a) Bile was collected from the common bile duct of 5-6 month old mice. Cholesterol (CH) secretory rates were derived from cholesterol concentrations and normalized to body weight (BW, *p=0.003, n=4-5 per group). (b) Relative lipid compositions (mol per 100 mol) of gallbladder bile obtained from Lox (open circles) and LIRKO (closed triangles) mice fed a chow diet were calculated and plotted on a partial condensed phase diagram (see Methods). The different phases are indicated for equilibrium conditions: micelles only (yellow); micelles and liquid crystals (orange); micelles, liquid crystals and cholesterol monohydrate crystals (red); and micelles and cholesterol monohydrate crystals (gray). Relative lipid compositions of bile from control mice plot in a zone in which only micelles would form at equilibrium, but bile from LIRKO mice plot in zones in which liquid crystals (orange) or both liquid crystals and cholesterol monohydrate crystals (red) in addition to micelles would form at equilibrium. (c) Cholesterol Saturation Indices (CSI) of gallbladder bile obtained from 5-6 month old mice were calculated as described in Methods. (d) Gallbladder volumes from 5-6 month old mice. Each point represents a single mouse, whereas long and short lines represent mean and SEM, respectively (*p=0.004). (e) Cholesterol absorption was measured in 4 month old mice by a fecal dual isotope ratio method. (f) Hepatic lipids were extracted from 2-3 month old Lox and LIRKO mice and total cholesterol content was measured by gas chromatography (n=4-6, *p<0.05). (g) Real-time PCR analysis of RNA isolated from the livers of non-fasted 8-10 week old mice (n=8, *p<1 x 10^-5). (h) Membranes purified from 8-10 week old mice were subjected to immunoblotting with antibodies against ABCG5 or ABCG8, and quantified with ImageJ software (*p<0.05, n=3-4).