Skip to main content
. 2009 May 29;41(9):629–637. doi: 10.3858/emm.2009.41.9.069

Figure 4.

Figure 4

Inhibitory effects of Tat-SOD on NF-κB activation, IκBα degradation and MAPK activation in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were treated with Tat-SOD or SOD fusion proteins for 1 h and then stimulated with LPS (100 ng/ml) for 30 min. (A) DNA-binding activity of NF-κB in the nuclear extracts of the RAW 264.7 cells was measured by EMSA. (B) Cell lysates were prepared from the RAW 264.7 cells treated with LPS and the levels of IκBα were determined by immunoblot analysis. (C) RAW 264.7 cells were incubated with Tat-SOD or SOD fusion protein for 1 h and then stimulated with LPS (100 ng/ml) for 30 min. Cell lysates were prepared and analyzed for MAP kinase protein activation by immunoblot using phospho-specific antibodies against MAPK proteins.