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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2009 Jul 22;240(2):143–148. doi: 10.1016/j.taap.2009.07.015

Figure 1.

Figure 1

Effect of chlorpyrifos oxon on tubulin polymerization visualized by atomic force microscopy. A) 0.1 mM bovine tubulin in PEM buffer, no GTP; B) 0.1 mM tubulin polymerized with 1 mM GTP, no chlorpyrifos oxon; C) 0.1 mM tubulin pretreated with 0.005 mM chlorpyrifos oxon before polymerization with GTP; D) pretreated with 0.010 mM chlorpyrifos oxon before polymerization; E) pretreated with 0.025 mM chlorpyrifos oxon before polymerization; F) pretreated with 0.050 mM chlorpyrifos oxon before polymerization; G) 0.1 mM tubulin pretreated with 0.1 mM chlorpyrifos oxon before polymerization; H) polymerized in the presence of 0.025 mM colchicine. The dimensions of each panel are 20 × 20 μm. Images are presented in height trace mode.