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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Stroke. 2009 Aug 6;40(10):3369–3377. doi: 10.1161/STROKEAHA.109.549212

Figure 2. Measurement of cross-sectional areas of ventricle, cortex and whole forebrain.

Figure 2

(A, B): Nissl’s stained cryosections at 2 coronal levels---mid-septal nucleus (upper panel) and ventro-lateral nucleus of thalamus (lower panel): —were evaluated. The cross-sectional areas of ventricle, cortex and whole forebrain were compared. Data are shown as box-and-whisker plot that depicts median, 10th,25th,75th and 90th percentile as vertical boxes with error bars (n=12 each). Ventricular area was significantly greater in IVH pups than saline- and glycerol-treated non-IVH controls at both the coronal levels. However, cerebral cortex and forebrain cross-sectional area were similar between the 3 groups.*P<0.05 for IVH pups vs. glycerol controls. #P<0.05 for IVH pups vs. saline controls. Greater gliosis in pups with IVH than controls (C,D): C) Representative immuno-fluorescence of cryosections labeled with GFAP antibody. Hypertrophic astrocytes (arrowhead) were abundant forming dense network in IVH pups, while non-pups had fewer astrocytes. D) Bar graph shows mean ± s.e.m.(n=6pups). Astrocyte count revealed significantly higher density in the periventricular zone (PVZ) and superficial WM of pups with IVH compared to glycerol- and saline-treated non-IVH controls, but not in the cerebral cortex. *P<0.05 for IVH pups vs. glycerol-treated non-IVH pups. #P<0.05 for IVH pups vs. saline-treated non-IVH pups.