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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Bone. 2009 Aug 6;45(5):1017–1023. doi: 10.1016/j.bone.2009.07.076

Fig. 1.

Fig. 1

Experimental setup for FRAP measurements of tracer diffusion in intact murine tibiae. (A) The FRAP experiment was performed using a Zeiss LSM 510 confocal module (not shown in the picture) attached to an Axioobserver Z1 invert microscope (1). A lens inverter (2) was used to direct the imaging laser beam to an elevated platform (3) beside the microscope, which was consisted of a height-adjustable jack and a xy table. The animal was placed in a water bath (4) perfused with 25°C PBS solution (heating elements not shown). (B) The left tibia was fastened rigidly at the knee using a rod with a cup-shaped end (5) and at the ankle using a slit and a rubber band (6) under a small compression force. The tibial anterior-medial surface (7) was surgically exposed for FRAP imaging.