Table 1.

The fluorescent tracers used for diffusion measurements within the bone LCS in situ

tracer	MW (Da)	shape	dye/tracer ratio (mol/mol)	[C] (mg/ml)	Vol. (ml)	dye in blooda (mM)	circulation time (hr)
sodium fluorescein	376	spherical	1	10	0.2	1.662	0.5
dextran-3k	3,000	Linear	1	10	0.5	0.477	1.5
(fluorescein)
dextran-10k	10,000	Linear	1	10	0.5	0.143	3
(fluorescein)
parvalbumin	12,300	Globular	2.2	5	0.2	0.056	1.5
(Alexa Fluor 488)
ovalbumin	43,000	Globular	4	10	0.5	0.134	7
(Texas Red)

^aNotes: The calculations were based on a blood volume of 3 ml for a mouse of 30g body weight.