. Author manuscript; available in PMC: 2010 Nov 1.

Published in final edited form as: Bone. 2009 Aug 6;45(5):1017–1023. doi: 10.1016/j.bone.2009.07.076

Table 2.

FRAP Measurements of diffusion coefficients for tracers in the bone LCS

Tracer	Number of measurements	A (µm²)	V_s (µm³)	N	d (µm)	V_r	D (µm²s⁻¹)
fluorescein	6	82±23	395±182	11±3	20±3	0.03±0.01	294.8±45.8
dextran-3k	11	65±23	271±133	9±3	21±3	0.03±0.01	128.0±31.7^a
parvalbumin	13	97±52	528±394	13±7	25±4	0.03±0.01	157.4±87.7^a
ovalbumin	17	94±28	450±193	13±4	26±5	0.04±0.01	65.3±20.7

Notes: Dextran-10k was not reported here because no recovery was found. Data were reported as mean ± standard deviation. No significant difference was found for A, V_s, N, d, and V_r.

No significant difference was found for D between Dextran-3k and parvalbumin (p>0.05), while the other groups differed significantly among each other (p< 0.05).

Definitions of Keys: A = cross-sectional area of the photobleached lacuna; V_s = total surface area of the photobleached lacuna; N = contributing canaliculi number (22% of the total canaliculi emanating from the lacuna at a number density of 0.18/µm²)[23]; d = average canalicular length; V_r = fluid volume ratio between contributing canaliculi and lacuna; D = diffusion coefficient of tracer in the bone LCS.