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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Dev Biol. 2009 Jul 28;334(2):535–546. doi: 10.1016/j.ydbio.2009.07.034

Table 1. unc-62 genetically interacts with ceh-20 and mab-5 to regulate M lineage development.

Genotype1 hlh-8∷gfp expression Early M lineage phenotypes2 SM lineage phenotypes3
Wild-type bright (100%) n=200 2 CCs, 2 SMs, 14 BWMs (100%) n=200 4 VM1s (100%) n=200
ceh-20(ay9) bright (80%) faint4 (20%) n=200 2 CCs, 2 SMs, 14 BWMs (91%) 1-3 CCs, 1 SM & 13-14 BWMs (9%) n=2005 4 VM1s (44%) >4 VM1s (56%) n=75
unc-62(e644) dpy-11(e224) bright (100%) n=200 2 CCs, 2 SMs, 14 BWMs (78%) 0-1 CCs, 3-4 SMs, 12-14 BWMs (22%) n=2006 4 VM1s (44%) >4 VM1s (56%) n=69
unc-62(ku234) bright (100%) n=200 2 CCs, 2 SMs, 14 BWMs (100%) n=2007 >4 VM1s (100%) n=200
mab-5(RNAi) no early expression bright SM lineage expression (100%) n=156 2 CCs, 2 SMs, 14 BWMs (6%) 0 CCs, 3-5 SMs, 13-14 BWMs (94%) n=156 4 VM1s (6%) >4 VM1s (94%)
ceh-20(ay9)/+; unc-62(e644) dpy-11(e224) bright (100%) n=123 2 CCs, 2 SMs, 14 BWMs (55%) 0-1 CCs, 3-4 SMs (45%) n=60 4 VM1s (7%) >4 VM1s (93%) n=123
ceh-20(ay9); unc-62(e644) dpy-11(e224) faint (3%) no expression (97%) N=200 0-1 CCs8 (100%) n=83 >4 VM1s (100%) n=45
ceh-20(ay9)/+; unc-62(ku234)/+ bright (100%) n=23 2 CCs, 2 SMs, 14 BWMs (100%) n=23 4 VM1s (100%) n=23
ceh-20(ay9)/+; unc-62(ku234) bright (100%) n=163 2 CCs, 2 SMs, 14 BWMs (72%) 0-1 CCs, 2 SMs (28%) n=163 >4 VM1s (100%) n=163
ceh-20(ay9); unc-62(ku234) faint (7%) no expression (93%) N=200 2 CCs, 2 SMs, 14 BWMs (13%) 0-1 CCs, 9-14 BWMs9 (87%) n=200 >4 VM1s (100%) n=200
mab-5(RNAi); unc-62(e644) dpy-11(e224) no expression (41%) bright in SM lineage10 (59%) n=200 2 CCs (1%) 0-1 CCs8 (99%) n=200 0 VM1s11 (58%) 4 VM1s (1%) >4 VM1s (41%) n=200
mab-5(RNAi) ceh-20(ay9) no expression (52%) bright in SM lineage10 (48%) n=200 2 CCs (9%) 0-1 CCs8 (91%) n=200 0 VM1s11 (60%) 4 VM1s (9%) >4 VM1s (31%) n=200
1

All animals examined carried the following GFP markers for the M lineage: intrinsic CC∷gfp(ccIs4438) III, ayIs2(egl-15∷gfp) IV; hlh-8∷gfp(ayIs6) X.

2

Phenotypes focus on specification of the 18 M lineage descendants (14 BWMs, 2 CCs and 2 SMs) produced at the L1 stage.

3

The SM lineage phenotypes shown here focused on the number of egl-15∷gfp expressing VM1-like cells.

4

hlh-8∷gfp signal was faint in both the early M lineage and the SM lineage.

5

The number of BWMs was obtained via examining 6 animals.

6

The number of BWMs was obtained via examining 5 animals.

7

The number of BWMs was obtained via examining 3 animals.

8

The number of BWMs and SMs was not determined in this mutant strain.

9

The number of BWMs was obtained via examining 5 animals.

10

There was no hlh-8∷gfp expression in the early M lineage.

11

Some of the animals had 1-3 elongated egl-15∷gfp positive cells randomly located in the posterior of the worm.