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. Author manuscript; available in PMC: 2010 Oct 9.
Published in final edited form as: Vaccine. 2009 Aug 4;27(43):6054–6059. doi: 10.1016/j.vaccine.2009.07.067

Table 1.

Determination of optimal OPA assay incubation time

Nominal concentration (μg/mL) a Experimentally determined Plate Incubation Time (minutes)
0 2 5 10 15
160 Concentration b 154.9 ± 5.0 160.2 ± 3.5 161.3 ± 3.2 158.5 ± 1.9 156.6 ± 4.4
% Accuracy c 98.8 ± 3.1 98.4 ± 0.2 98.6 ± 1.1 99.0 ± 1.2 97.9 ± 2.7
120 Concentration 116.4 ± 7.7 119.9 ± 3.7 122.3 ± 4.4 119.4 ± 4.5 118.3 ± 7.3
% Accuracy 95.5 ± 4.3 97.8 ± 0.1 97.4 ± 2.7 97.3 ± 0.7 95.7 ± 2.0
70 Concentration 67.9 ± 0.5 70.6 ± 0.6 70.9 ± 1.2 70.5 ± 0.8 71.1 ± 1.6
% Accuracy 97.1 ± 0.7 99.1 ± 0.9 98.8 ± 1.7 99.2 ± 1.0 98.4 ± 2.3
40 Concentration 36.8 ± 2.8 38.3 ± 0.5 39.4 ± 1.3 38.6 ± 1.4 39.6 ± 5.1
% Accuracy 92.0 ± 7.1 95.8 ± 1.4 97.7 ± 2.2 96.6 ± 3.4 91.0 ± 1.4
20 Concentration 16.3 ± 0.6 16.8 ± 0.8 17.3 ± 0.2 16.7 ± 0.1 18.9 ± 3.7
% Accuracy 81.4 ± 3.0 83.9 ± 4.1 86.3 ± 1.0 83.7 ± 0.3 86.8 ± 8.1
10 Concentration 4.9 ± 2.2 5.6 ± 0.3 6.0 ± 0.5 5.6 ± 0.3 8.8 ± 3.7
% Accuracy 48.6 ± 21.8 55.5 ± 3.5 60.2 ± 5.3 55.6 ± 3.4 74.1 ± 16.9
a

Known amount of MSP142-3D7 was freshly prepared on Alhydrogel®.

b

Concentrations were back calculated by using linear regression of the standard curve.

c

Percent accuracy is percent similarity between the amount of protein calculated and the known amount of protein added. Percent accuracy = (|calculated concentration − nominal concentration|/nominal concentration) × 100.