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. Author manuscript; available in PMC: 2010 Oct 16.
Published in final edited form as: J Mol Biol. 2009 Aug 13;393(1):191–201. doi: 10.1016/j.jmb.2009.08.008

Figure 2.

Figure 2

Correlation of loop truncation with quaternary structure changes. A) Sequence alignment of N and C-terminal region of CALB. The original variant cp283 carries a six-amino acid linker (grew box) and has its new N-terminus moved to position 283. Subsequent incremental truncation of cp283, followed by screening for functional lipases, yields a series of variants (cp283Δ2–11) with shortened linker regions. Representatives with various linker lengths were selected for overexpression and in vitro characterization (indicated by labels). B) Size exclusion chromatograms for wild type CALB, cp283, and truncated variants. While CALB and cp283 are monomeric (RCV ~0.81, estimated MW: 32 kDa), a second peak at RCV ~0.72, corresponding to dimer, emerges in variants with truncated loops. The third peak (RCV ~0.68) in cp283Δ8 like represents the trimer form. Molecular weights were calculated based on the calibration curve with protein size standards (data not shown).