Figure 3.

Deletion of the 62 bp region from the let-7 rescue fragment causes an increase in precocious alae expression. (A) At the eL4 stage, wild-type (N2) animals do not express alae, while wild-type animals injected with the wild-type rescue fragment express precocious alae at a low level. Significantly more wild-type animals injected with a rescue fragment that has the 62 bp region deleted show precocious alae. n ≥ 30 for each strain. Three independent lines were analyzed for each construct. (B) Nomarski DIC images of wild-type eL4 C. elegans showing that no alae are expressed. (C) Nomarski DIC image showing the stage of the animal in (B). (D) Nomarski DIC images of wild-type eL4 C. elegans injected with the let-7 rescue fragment which has the 62 bp region deleted showing precociously expressed alae. Arrows indicate alae. (E) Nomarski DIC image showing the stage of the animal in (D). (F) Wild-type eL4 animal fed 50% hbl-1(RNAi) which shows precocious alae formation similar to precocious alae seen in C. elegans injected with the 62 bp deleted rescue fragment. Arrows indicate alae. Precocious alae in hbl-1(lf) has previously been identified in other studies (Abrahante et al., 2003; Lin et al., 2003). (G) Nomarski DIC image showing the stage of the animal in (F). Scale bars all represent 20 microns. Anterior is left and dorsal is top on all images.