Fig. 2.

Raman spectrum of HDV ribozyme crystals. The spectrum of the wild-type ribozyme (using 2′-OCH₃ substitution at the U-1 position of the inhibitor strand) in the region of 1800–600 cm⁻¹. The crystal was surrounded by cacodylate buffer containing 20 mM Mg²⁺ at pH 7.0 at room temperature (22 °C). The frequencies of major Raman bands are marked in the spectrum and assignments of these bands are shown in Table 3. The vertical bar represents a 5000 photon event, and the y-axis scales linearly with the number of photons. Very high signal-to–noise ratios are obtained, ≈ 300:1. Reproduced with permission from [24]. (Copyright, American Chemical Society.)