Table 4.
Positions (cm−1) and assignment of major Raman difference bands observed in Raman difference spectra of [HDV pH 7.5] minus [HDV pH 5.0] and [CMP pH 6.0] minus [CMP pH 3.0]
| HDV | CMP | ||
|---|---|---|---|
| Wavenumber (cm−1) | Assignment | Wavenumber (cm−1) | Assignment |
| 1547 | C+ | ||
| 1528 | C | 1529 | C |
| 1489/1473 | G | ||
| 1341 | A | ||
| 1298 | C | 1298 | C |
| 1258 | C+ | 1258 | C+ |
| 983 | PO32− | ||
| 929 | ribose | ||
| 916/896 | ribose | ||
| 892 | ribose | ||
| 815 | -O-P-O- | ||
| 790 | C+ | 790 | C+ |
| 780 | C | 778 | C |
| 728 | A | ||
| 599 | C | 599 | C |
| 323 | Mg(H2O)x2+ (≤5) | ||
C, neutral cytosine; C+, protonated cytosine; Mg(H2O)x2+ (x≤5), inner-sphere coordinated Mg-hydrate. The features assigned to the bases are due to ring modes. Reproduced with permission from [26]. (Copyright, American Chemical Society.)