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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Circ Res. 2008 Aug 7;103(6):591–597. doi: 10.1161/CIRCRESAHA.108.173963

Figure 3. Identification of the eNOS 3'-UTR binding site in eEF1A1.

Figure 3

(A) Schematic representation of human eEF1A1 functional domains. (B) Identification of GST-fusion proteins containing different domains of eEF1A1. GST-fusion proteins were bacterially expressed and purified by glutathione-Sepharose beads. Purified proteins were separated on 12% SDS-PAGE and stained with Coomassie Blue R250. (C) UV cross-linking showing the binding of eEF1A1 domain III (D3) to the human eNOS 3'-UTR. UV cross-linking assays were performed by incubating the radiolabeled eNOS 3'-UTR with equal molar of GST fusion proteins.