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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Immunol Cell Biol. 2008 Dec 16;87(3):209–217. doi: 10.1038/icb.2008.101

Figure 1. Brefeldin A inhibits TLR9 induced NF-κB activity.

Figure 1

(a) HEK293 cells were transfected with TLR9 and pre-treated with the indicated concentration of BFA for 1 hour prior to overnight stimulation with 1 µg/ml CpG DNA or 100 ng/ml flagellin. (Error bars=mean+/−standard deviation of triplicate samples) Data are representative of three experiments. (b) Similar to (a) except that the cells were pre-treated for the indicated time period with 30 ng/mL BFA. (Error bars=mean+/−standard deviation of triplicate samples) Data are representative of three experiments.