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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Immunol Cell Biol. 2008 Dec 16;87(3):209–217. doi: 10.1038/icb.2008.101

Figure 5. TLR9 trafficking to endolysosomes requires Golgi transport.

Figure 5

(a) Fractions from BJAB cells separated on Percoll-sucrose gradients were immunoblotted for early endosomes (Rab 5), ER (Calnexin), lysosomes (LAMP-1) and TLR9. Fractions were run on three gels, which are indicated with boxes over the fraction numbers, but were immunoblotted and developed simultaneously. (b) Each immunoblot in (a) was quantified to determine absolute intensity. Percent of maximum values were calculated from the absolute intensities and graphed on the same graph to normalize the data. Data are representative of three experiments. (c) Similar to (b) except that the cells were HEK293 cells stably expressing HAfu-TLR9 (upper) or HA-TLR9 (lower) and were additionally immunoblotted for HA. Data are representative of three experiments. (d) HA immunoprecipitates from combined fractions that were LAMP-1 (Lys, fractions 17–20) or Rab 5 (EE, fractions 1–4) positive collected from HA-TLR9 cells were immunoblotted for TLR9, stripped and probed with biotinylated DS lectin. Data are representative of two experiments.