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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Anal Biochem. 2003 Apr 1;315(1):57–66. doi: 10.1016/S0003-2697(02)00702-9

Table 2.

Multiexponential analysis of DNA(Cy5)-biotin intensity decays

Conditions k τ〉 (ns) τ ¯(ns) αi fi τ (ns)
χR2
Quartz 1a 1.00b 1.40c 0.369 0.085 0.22
0.604 0.915 1.51 0.9
2 1.17 1.42 0.252 0.051 0.23
0.748 0.949 1.48 0.9
3 1.25 1.40 0.173 0.044 0.32
0.827 0.956 1.45 1.7
4 1.35 1.43 0.155 0.064 0.56
0.845 0.936 1.49 1.5
5 1.37 1.46 0.139 0.051 0.50
0.861 0.949 1.51 0.9
6 1.49 1.63 0.147 0.041 0.42
0.853 0.959 1.68 1.8
Silver 1 0.057 0.392 0.822 0.204 0.014
0.160 0.479 0.168
0.018 0.317 0.975 1.7
2 0.152 0.626 0.716 0.174 0.037
0.231 0.399 0.263
0.053 0.427 1.209 1.0
3 0.266 0.830 0.467 0.064 0.036
0.406 0.335 0.218
0.127 0.601 1.256 1.8
4 0.428 1.009 0.427 0.067 0.067
0.358 0.230 0.275
0.215 0.703 1.400 1.9
5 0.446 1.030 0.498 0.105 0.094
0.288 0.224 0.348
0.214 0.671 1.406 1.0
6 0.691 1.473 0.457 0.038 0.057
0.191 0.109 0.396
0.352 0.853 1.675 0.9
a

Number of BSA-biotin-avidin layers.

b

τ〉 = Σαiτi.

c

τ̄ = Σ fiτi, fi=αiτiαiτi.