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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Cell Physiol. 2009 Jun;219(3):659–666. doi: 10.1002/jcp.21712

FIGURE 3. Involvement of Cripto-1/c-Src Kinase - Nodal independent pathway in Msx2-induced EMT.

FIGURE 3

(a). Expression levels of TGF-β1, TGF-β3, TGF-β-Receptor II, Twist, Snail, Slug and Cripto were detected by RT-PCR in NMuMG-EV (EV) and NMuMG-Msx2 (Msx2) total RNA preparations. (b) Cripto-1 expression level was detected in NMuMG-EV (EV) and NMuMG-Msx2 (Msx2) cell lysates by western blot analysis and by immunocytochemistry on fixed NMuMG-EV (EV) and NMuMG-Msx2 (Msx2) cells. Nuclei were stained with DAPI. (c) Nodal expression level was detected by RT-PCR in NMuMG-EV (EV) and NMuMG-Msx2 (Msx2) total RNA preparations. The PCR positive control for Nodal (ES) was performed using total RNA extracted from mouse embryonic stem cells. (d) The active c-Src kinase pathway was determined by western blot analysis of phosphorylated c-Src, FAK, Akt and Erk using specific antibodies. Total c-Src, FAK, Akt and Erk were also determined by western blot.

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