FIGURE 5. Reduction of Cripto expression or incubation with c-Src inhibitor SU6656 are able to revert Msx2-induced EMT in NMuMG cells overexpressing Msx2.

(a) NMuMG-Msx2 were transiently transfected with siRNA for a non-target Control (Msx2+siRNA Control) or with siRNA for m-Cripto-1 (Msx2+siRNA Cripto): 48h post- transfection, cell lysates were prepared and western blot analysis were performed for the EMT markers E-Cadherin, Vimentin and N-Cadherin. (b) NMuMG-Msx2 cells were incubated in culture with DMSO (vehicle control), Alk4/5/7 inhibitor SB-431542 (10μM) or c-Src inhibitor SU6656 (10μM). After 3 or 7 days of incubation, cell lysates were prepared and western blot analysis were performed for EMT markers E-Cadherin, Vimentin and N-Cadherin. (c) NMuMG-Msx2 cells were incubated in culture with different concentrations of c-Src inhibitor SU6656 (1μM, 5μM and 10μM). After 4 days of incubation, cell lysates were prepared and western blot analyses were performed for the EMT markers E-Cadherin, Vimentin and N-Cadherin.