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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Arch Biochem Biophys. 2008 Dec 10;484(2):205–213. doi: 10.1016/j.abb.2008.11.027

Figure 5. Cytokine-induced TER loss in MTE cells is independent of NOS2.

Figure 5

MTE cells from C57Bl6/J (A) or NOS2-/- mice (B) were treated with a cytokine mixture (CM: 10 ng/mL TNF-α; 1 ng/mL IL-1β; 100 ng/mL IFN-γ) and TER was monitored over 24 hrs. Where indicated, the NOS2-specific inhibitor 1400W (100 μM) was added 30 min prior to CM stimulation. Data were expressed relative to initial TER for each well (n=4). Effects of CM stimulation on NOS2 mRNA expression or NO• production was assessed by RT-PCR (C) or analysis of nitrite accumulation in the media (D). White bars = control; Black bars = cytokine mix; Grey bars = cytokine mix + 1400W.