Skip to main content
. Author manuscript; available in PMC: 2010 Sep 29.
Published in final edited form as: Biochemistry. 2009 Sep 29;48(38):9076–9083. doi: 10.1021/bi9006385

FIGURE 2.

FIGURE 2

Growth phenotypes of R. capsulatus strain SBI/II− complemented with wild-type and mutant Synechococcus Rubisco enzymes. (A) anaerobic CO2-dependent photoautotrophic growth under an atmosphere of 5–10 % CO2/90–95 % H2; (B) anaerobic CO2-dependent photoautotrophic growth under an atmosphere of 1.5 % CO2/98.5 % H2; (C) aerobic CO2-dependent chemoautotrophic growth under an atmosphere of 5–10 % CO2/40–45 % H2/50 % air; and (D) aerobic mixotrophic growth on a complex peptone-yeast extract medium under an atmosphere of 5–10 % CO2/40–45 % H2/50 % air as previously described (1). In all four plates, sector 1 contains wild-type R. capsulatus strain SB1003 with its endogenous form I and form II Rubisco genes that allow growth under all conditions (36); sector 2 contains R. capsulatus strain SBI/II− lacking endogenous functional Rubisco genes; sectors 3 to 6 contain R. capsulatus strain SBI/II− with plasmids containing the wild-type Synechococcus rbcLrbcS genes (sector 3), rbcL-D103V/rbcS (sector 4), rbcL-A375V/rbcS (sector 5), and rbcL-D103V/ A375V/rbcS (sector 6).