Klf-9 loss of function does not affect passive membrane properties and action potential characteristics. A–C, Passive membrane properties as measured by whole-cell voltage recordings from DG granule neurons (P14–P17) are not different between Klf-9+/+ and Klf-9−/− mice. A, Capacitance of DG granule neurons recorded from Klf-9+/+ versus Klf-9−/− mice (57.91 ± 5.26 pF vs 57.66 ± 4.44 pF); p = 0.98, unpaired t test. B, Impedance of DG granule neurons recorded from Klf-9+/+ versus Klf-9−/− mice (421.55 ± 33.39 MΩ vs 466.24 ± 35.26 MΩ); p = 0.36, unpaired t test. C, Time constant of DG granule neurons recorded from Klf-9+/+ versus Klf-9−/− mice (19.15 ± 1.24 ms vs 21.33 ± 1.07 ms); p = 0.19, unpaired t test. D–F, Action potential characteristics. D, Action potential width: 2.54 + 0.1 ms (Klf-9+/+) versus 2.62 + 0.15 ms (Klf-9−/−). E, Action potential threshold of DG granule neurons recorded from Klf-9+/+ versus Klf-9−/− mice (26.36 ± 1.20 mV vs 24.85 ± 1.28 mV); p = 0.4, unpaired t test. F, Action potential amplitude of DG granule neurons recorded from Klf-9+/+ versus Klf-9−/− (68.77 ± 2.93 mV vs 68.53 ± 3.35 mV); p = 0.92, unpaired t test. n = 27 (control) and 30 (KO) cells. Results are mean ± SEM.