Table. Negligible Role of VEGF-B in Revascularization After Limb Ischemia.
| WT Mice | ||||||||
|---|---|---|---|---|---|---|---|---|
| WT Mice | VEGF-B−/− Mice | Empty Plasmid | pmVEGF-B167 | Ad.RR5 | Ad.hVEGF-B167 | |||
| Angiogenesis | ||||||||
| Capillary-to-myocyte ratio | 0.96±0.06 | 0.97±0.06 | ND | ND | 1.16±0.06 | 1.19±0.10 | ||
| Total limb perfusion | ||||||||
| Laser Doppler (% of non-ligated) | 90±5 | 90±11 | 86±9 | 82±6 | 92±5 | 94±4 | ||
| Treadmill exercise test | ||||||||
| Endurance (% of baseline) | 56±6 | 50±7 | ND | ND | ND | ND | ||
| Grade exercise (% of baseline) | 57±4 | 52±6 | ND | ND | ND | ND | ||
| Collateral growth | ||||||||
| Lumen area, μm2 | ||||||||
| Main collateral artery | 2810±300 | 2480±150 | 2890±190 | 2630±280 | 2294±425 | 2415±511 | ||
| 2nd collateral branch | 740±75 | 790±50 | 760±60 | 790±65 | 704±190 | 688±163 | ||
| 3rd collateral branch | 120±10 | 140±7 | 96±3 | 98±6 | 105±13 | 92±13 | ||
| Collateral side branches (n/mm2) | ||||||||
| 2nd collateral branches | 3±0.8 | 3±0.7 | 2±0.3 | 3±0.4 | 4±1.8 | 3±1.4 | ||
| 3rd collateral branches | 5±1.0 | 6±1.5 | 8±1.2 | 9±1.6 | 7±1.6 | 10±3.6 | ||
| Total perfusion area, μm2/mm2 | 2730±700 | 3000±800 | 3140±360 | 3460±670 | 3098±569 | 3020±524 | ||
Analysis was performed as described in methods. ND indicates not determined; P=NS for WT/control mice vs VEGF-B−/− /VEGF-B–treated animals.