Skip to main content
. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: J Immunol. 2009 Jul 27;183(4):2678–2687. doi: 10.4049/jimmunol.0803398

FIGURE 2.

FIGURE 2

Cytokine secretion by monocyte subsets in response to resting and germinated conidia. A, CD14+CD16− and CD14+CD16+ monocytes were purified from untreated donors or flow through fractions of G-CSF-mobilized donors (G-PB) and infected with heat-killed conidia, dormant conidia and conidia germinated for 6 h (germinated) at 1:1 conidia:cell ratio. Supernatants were collected 16 h later and TNF (A) and IL-1β (B) secretion was analyzed by ELISA. Each data point corresponds to a value obtained for an individual donor with three untreated and five to seven G-CSF treated donors analyzed. Shown are mean values. The unpaired Student t test was used to compare groups. Statistical analysis was performed with Prizm 5. A value of p = 0.05 was considered significant.