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. Author manuscript; available in PMC: 2010 Aug 25.
Published in final edited form as: Biochemistry. 2009 Aug 25;48(33):8044–8050. doi: 10.1021/bi900886g

Table 1.

Kinetic constants a for phosphotransfer reactions in the absence and presence of NaCl.

Reaction SLN1-R1∼P + YPD1 YPD1∼P + SSK1-R2
NaCl 0 M 0.2 M 0.6 M 1 Ma 0 M 0.2 M 0.6 M 1 Mb
kfwd (s−1) 27.0 ± 2.6 32.9 ± 1.8 24.6 ± 0.8 14.7 ± 0.8 110 ± 8 77.0 ± 0.2 41.0 ± 0.5 32.0 ± 3.2
krev (s−1) 9.2 ± 3.3 0.21 ± 2.1c 10.0 ± 0.9 20.7 ± 0.4 4.1 ± 1.4 0.8 ± 0.3 2.2 ± 0.7 6.1 ± 2.9
Kd (μM) 2.8 ± 0.8 2.3 ± 0.6 2.6 ± 0.4 6.2 ± 1.6 3.8 ± 1.5 2.2 ± 0.3 1.4 ± 0.1 3.0 ± 1.6
kfwd/Kd (μM−1s−1) 9.6 ± 2.9 14.3 ± 3.8 9.5 ± 1.5 2.4 ± 0.6 29 ± 4 35.0 ± 4.8 28.6 ± 2.0 10.6 ± 5.8
a

The data used to calculate the values in the Tables 1-3 obtained in triplicates and the errors represent standard errors of the mean.

b

1 M NaCl considered not physiologically relevant.

c

Undefined