Figure 1.

Fve stimulates mouse splenic T-cell activation in an accessory-cell-dependent manner. Purified CD90⁺ T cells (1 × 10⁵ cells/well) were co-cultured with mitomycin C-treated bone marrow-derived dendritic cells (BM-DCs) (2 × 10⁴ cells/well) in the presence or absence of Fve (20 μg/ml) in triplicate using the 96-well plate. The DCs and CD90⁺ T cells alone were included for comparison. The cultures were pulsed with [³H]thymidine for the last 18 hr of the co-culture. The cells were harvested and thymidine incorporation was measured by liquid scintillation counting at 72 hr (a). The culture supernatants were collected at 72 hr and interferon (IFN)-γ production was measured by enzyme-linked immunosorbent assay (ELISA) (b). *P < 0·05. c.p.m., counts per minute.