Figure 3.

Co-immunization with human papillomavirus (HPV)-16 E7 and Fve enhanced protection against the growth of TC-1 tumours. Mice (n= 10 per group) were immunized with phosphate-buffered saline (PBS) (♦), 20 μg of HPV-16 E7 (▪), 20 μg of Fve (×) or 20 μg of HPV-16 E7 plus 20 μg of Fve (▴) at days 0, 14 and 28 and then inoculated subcutaneously with TC-1 cells at day 30. The mice were monitored daily for tumour growth by palpation (a) and the tumour size was measured every 2 days (b). One hundred and sixty-seven days after tumour challenge, splenocytes were collected from the tumour-free mice and cultured with HPV-16 E7 protein in vitro. Supernatants were collected at 72 hr and interferon (IFN)-γ levels were analysed (c).