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. 2009 Sep;128(1 Pt 2):e881–e894. doi: 10.1111/j.1365-2567.2009.03099.x

Figure 8.

Figure 8

Fve greatly enhanced antigen-specific responses of CD8+ T cells. Mice (n= 8 mice/group) were intraveneously injected with phosphate-buffered saline (PBS), ovalbumin (OVA), Fve or OVA plus Fve and the dendritic cells (DCs) were isolated from the pool of eight spleens harvested from each group of mice 24 hr later. Purified DCs were pulsed with 1 μm OVA257–264 peptide or 1 μm OVA323–367 peptide for 2 hr at 37° and washed three times before co-culturing with CD8+ T cells from OT-I mice or CD4+ T cells from OT-II mice, respectively, for 72 hr. Supernatants were collected for cytokine assays by enzyme-linked immunosorbent assay (ELISA). The experiments were performed three times and representative data from one of these experiments are presented. DC, dendritic cells alone; DC:CD8 = 1 : 10, the ratio of DCs to CD8+ T cells from OT-I mice is 1 : 10; DC:CD4 = 1 : 10, the ratio of DCs to CD4+ T cells from OT-II mice is 1 : 10.